Availability
- Request Lead Time
- In stock and ready for quick dispatch
- Usually dispatched within 5-10 working days
Product Overview
| Product Name | IL1 beta antibody |
|---|---|
| Catalog Number | GRP177 |
| Species/Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Tested applications | ELISA, FA, ICC, IF, IHC-Fr, IHC-P, WB |
| Immunogen | Recombinant murine IL1 beta |
| Alternative Names | (click to expand) |
Product Properties
| Form/Appearance | Liquid: PBS |
|---|---|
| Concentration | 1.3 mg/ml |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Purity | Purified by antigen-affinity chromatography. |
| Uniprot ID | P10749 |
| Entrez | 16176 |
Product Description
IL1 beta antibody
Application Notes
| Dilution Range | WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000,ELISA: 0.5-2?g/ml |
|---|
Validation Images
An ELISA plate is coated with 50 μL of IL1 beta recombinant protein at concentration of 5 μg/mL. The coated protein is detected with anti-IL1 beta antibody (GRP629) at concentration ranged from 0.01 to 0.15 μg/mL.
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GRP629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the pri
IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.Sample: Frozen-sectioned mouse hippocampus.Green: IL1 beta stained by IL1 beta antibody (GRP629) diluted at 1:250.Blue: Fluoroshield with DAPI.
Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GRP629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Untreated (–) and treated (+) THP-1 whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GRP629) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used
Untreated (–) and treated (+) Raw264.7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody (GRP629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the pri
IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IL1 beta stained by IL1 beta antibody (GRP629) diluted at 1:500.
IL1 beta antibody detects IL1 beta protein at microglia by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Green: IL1 beta stained by IL1 beta antibody (GRP629) diluted at 1:250.Red: NF-H, a neuron marker, stained by NF-H antibody [G
Reviews
Write Your Own Review
