Glutamine synthetase antibody [GT1055]

Catalog Number: GRP173

Product Overview

Product Name	Glutamine synthetase antibody [GT1055]
Catalog Number	GRP173
Species/Host	Mouse
Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated
Tested applications	ICC, IF, IHC-Fr, IHC-P, WB
Immunogen	Full length human Glutamine synthetase Recombinant protein.
Alternative Names	(click to expand) glutamate-ammonia ligase \| GLNS \| GS \| PIG43 \| PIG59

Product Properties

Form/Appearance	Liquid: PBS
Concentration	1 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG1
Clonality	Monoclonal
Purity	Affinity purified by Protein G.
Clone ID	GT1055
Uniprot ID	P15104
Entrez	2752

Product Description

Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling (Haberle et al., 2005 [PubMed 16267323]). Fetal glutamine requirements are very high and depend largely on active glutamine synthesis and the release of glutamine into the fetal circulation by the placenta. Glutamine synthetase (EC 6.3.1.2), also called glutamate-ammonia ligase (GLUL), is expressed throughout the body and plays an important role in controlling body pH and in removing ammonia from the circulation. The enzyme clears L-glutamate, the major neurotransmitter in the central nervous system, from neuronal synapses (see references in Clancy et al., 1996 [PubMed 8975719]).[supplied by OMIM]

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000,IHC-Fr: 1:100-1:1000

Validation Images

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: Glutamine synthetase antibody [GT1055] (GRP625) diluted at

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatoma. Glutamine synthetase antibody [GT1055] (GRP625) dilution: 1:500.

Non-transfected (â€“) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GRP625) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody (GRP625) diluted at 1:1000.

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g HeLa whole cell lysate/extract C. 30 ?g HepG2 whole cell lysate/extract10 % SDS-PAGEGlutamine synthetase

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. Glutamine synthetase antibody [GT1055] (GRP625) dilution: 1:500.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GRP625) diluted at 1:5000.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [GT1055] (GRP625) diluted at 1:5000.

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Glutamine synthetase antibody [GT1055] (GRP625) dilution: 1:500.

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by western blot analysis.A. 30 ?g U87-MG whole cell lysate/extract B. 30 ?g SK-N-SH whole cell lysate/extract C. 30 ?g IMR32 whole cell lysate/extract D. 30 ?g SK-N-AS whole cell

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina.Green: Glutamine synthetase protein stained by Glutamine synthetase antibody [GT1055] (GRP625) diluted

Glutamine synthetase antibody [GT1055] detects Glutamine synthetase protein at cytoplasm by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamine synth

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