TDP43 antibody [GT225]

Catalog Number: GRP172

Product Overview

Product Name	TDP43 antibody [GT225]
Catalog Number	GRP172
Species/Host	Mouse
Reactivity	Human, Mouse, Rat
Conjugation	Unconjugated
Tested applications	ICC, IF, IHC-P, WB
Immunogen	Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.
Alternative Names	(click to expand) TAR DNA binding protein \| ALS10 \| TDP-43

Product Properties

Form/Appearance	Liquid: PBS
Concentration	1 mg/ml
Storage	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Note	For research use only.
Isotype	IgG2b
Clonality	Monoclonal
Purity	Affinity purified by Protein G.
Clone ID	GT225
Uniprot ID	Q13148
Entrez	23435

Product Description

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]

Application Notes

Dilution Range	WB: 1:500-1:3000,ICC: 1:100-1:1000,IHC-P: 1:100-1:1000

Validation Images

TDP43 antibody [GT225] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TDP43 antibody [GT225] (GRP624) diluted at 1:200.

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract10 % SDS-PAGETARDBP antibody [GT225] (GRP624) dilution:

TDP43 antibody [GT225] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. TDP43 antibody [GT225] (GRP624) diluted at 1:200.

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.A. 30 μg BCL-1 whole cell lysate/extract B. 30 μg Raw264.7 whole cell lysate/extract10 % SDS-PAGETARDBP antibody [GT225] (GRP624) dilution: 1:1000

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.A. 30 μg PC-12 whole cell lysate/extractB. 30 μg Rat2 whole cell lysate/extract10 % SDS-PAGETARDBP antibody [GT225] (GRP624) dilution: 1:1000

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT225] (GRP624) diluted at 1:500.

TDP43 antibody [GT225] detects TDP43 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nestin stained by Nestin antibody (GRP624) diluted at 1:500.Red: TDP43

TDP43 antibody [GT225] detects TDP43 protein immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nestin stained by Nestin antibody (GRP624) diluted at 1:500.Red: TDP43 st

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